SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB06737

Signal sequence   MKKKPLFRTFMCAALIGSLLAPVAVQADTGTA

Length  32

Organism  Bacillus licheniformis strain 3F-3

UniProt ID  NA,

Motif  1..32



Activity Information


Pathway  NA

Natural/Artificial  Artificial

Subcellular location  Extracellular environment

Secretion efficiency  Nattokinase yield: 22.68(FU/mL)

Type  NA



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB06737.cif

Structure  SPKB06737.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  3351.08724

Aliphatic index  97.8125

Isoelectric point  10.50672576

Charge (pH=7)   2.935567655

Topological polar surface area  1249.11

Aromaticity  0.0625

Average rotatable bonds  3.40625

Number of hydrogen bond donors  44

Number of hydrogen bond acceptors  46

X logP energy  -10.18923

Hydropathy 



Literature Information


Title  High-level expression of nattokinase in Bacillus licheniformis by manipulating signal peptide and signal peptidase

DOI  10.1111/jam.13175,

Publication Year  2016

PubMed ID  27159567,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.