General Information
SPKB ID SPKB06735
Signal sequence MRKWYFILSACILVSVIIAFA
Length 21
Organism Bacillus licheniformis strain 3F-3
UniProt ID NA,
Motif 1..21
Activity Information
Pathway Sec
Natural/Artificial Artificial
Subcellular location Extracellular environment
Secretion efficiency Nattokinase yield: 27.97(FU/mL)
Type Sec/SPI
Structure Information
PDB ID NA,
Predicted Structure ID SPKB06735.cif
Physicochemical Information
Molecular weight 2445.06504
Aliphatic index 153.3333333
Isoelectric point 9.447869836
Charge (pH=7) 1.934860824
Topological polar surface area 809.72
Aromaticity 0.19047619
Average rotatable bonds 3.666666667
Number of hydrogen bond donors 31
Number of hydrogen bond acceptors 29
X logP energy -0.03993
Hydropathy 
Literature Information
Title High-level expression of nattokinase in Bacillus licheniformis by manipulating signal peptide and signal peptidase
DOI 10.1111/jam.13175,
Publication Year 2016
PubMed ID 27159567,
Patent ID NA,
