General Information
SPKB ID SPKB06734
Signal sequence MLRSQRTKKKRLRKWVKYSLFFIALILTATA
Length 31
Organism Bacillus licheniformis strain 3F-3
UniProt ID NA,
Motif 1..31
Activity Information
Pathway Sec
Natural/Artificial Artificial
Subcellular location Extracellular environment
Secretion efficiency Nattokinase yield: 31.72(FU/mL)
Type Sec/SPI
Structure Information
PDB ID NA,
Predicted Structure ID SPKB06734.cif
Physicochemical Information
Molecular weight 3768.65794
Aliphatic index 107.0967742
Isoelectric point 12.54041985
Charge (pH=7) 8.995645471
Topological polar surface area 1494.28
Aromaticity 0.129032258
Average rotatable bonds 4.225806452
Number of hydrogen bond donors 57
Number of hydrogen bond acceptors 49
X logP energy -9.01792
Hydropathy 
Literature Information
Title High-level expression of nattokinase in Bacillus licheniformis by manipulating signal peptide and signal peptidase
DOI 10.1111/jam.13175,
Publication Year 2016
PubMed ID 27159567,
Patent ID NA,
