General Information
SPKB ID SPKB06733
Signal sequence MRSKKLWISLLFALTLIFTMAFSNMSA
Length 27
Organism Bacillus licheniformis strain 3F-3
UniProt ID NA,
Motif 1..27
Activity Information
Pathway Sec
Natural/Artificial Artificial
Subcellular location Extracellular environment
Secretion efficiency Nattokinase yield: 31.99(FU/mL)
Type Sec/SPI
Structure Information
PDB ID NA,
Predicted Structure ID SPKB06733.cif
Physicochemical Information
Molecular weight 3121.85074
Aliphatic index 112.2222222
Isoelectric point 11.82305527
Charge (pH=7) 2.99739092
Topological polar surface area 1114.12
Aromaticity 0.148148148
Average rotatable bonds 3.851851852
Number of hydrogen bond donors 41
Number of hydrogen bond acceptors 41
X logP energy -5.77563
Hydropathy 
Literature Information
Title High-level expression of nattokinase in Bacillus licheniformis by manipulating signal peptide and signal peptidase
DOI 10.1111/jam.13175,
Publication Year 2016
PubMed ID 27159567,
Patent ID NA,
