General Information
SPKB ID SPKB06730
Signal sequence MVSIRRSFEAYVDDMNIITVLIPAEQKEIM
Length 30
Organism Bacillus subtilis WB600
UniProt ID NA,
Motif 1..30
Activity Information
Pathway Tat
Natural/Artificial Artificial
Subcellular location Periplasmic space
Secretion efficiency Alkaline polygalacturonate lyase yield: 16(U/ml)
Type Tat/SPI
Structure Information
PDB ID 2E8Y,
Predicted Structure ID SPKB06730.cif
Physicochemical Information
Molecular weight 3513.14294
Aliphatic index 113.6666667
Isoelectric point 4.264691876
Charge (pH=7) -1.996949435
Topological polar surface area 1401.85
Aromaticity 0.066666667
Average rotatable bonds 3.966666667
Number of hydrogen bond donors 48
Number of hydrogen bond acceptors 48
X logP energy -9.59596
Hydropathy 
Literature Information
Title High-level extracellular production of alkaline polygalacturonate lyase in Bacillus subtilis with optimized regulatory elements
DOI 10.1016/j.biortech.2013.07.129,
Publication Year 2013
PubMed ID 23973973,
Patent ID NA,
