General Information
SPKB ID SPKB06729
Signal sequence MKRIRIPMTLALGAALTIAPLSFASA
Length 26
Organism Bacillus subtilis WB600
UniProt ID O32211,
Motif 1..26
Activity Information
Pathway Sec
Natural/Artificial Artificial
Subcellular location Extracellular environment
Secretion efficiency Alkaline polygalacturonate lyase yield: 21.6(U/ml)
Type Sec/SPI
Structure Information
PDB ID NA,
Predicted Structure ID SPKB06729.cif
Physicochemical Information
Molecular weight 2714.36664
Aliphatic index 128.0769231
Isoelectric point 12.51647815
Charge (pH=7) 2.997682643
Topological polar surface area 1003.98
Aromaticity 0.038461538
Average rotatable bonds 3.346153846
Number of hydrogen bond donors 36
Number of hydrogen bond acceptors 36
X logP energy -7.24516
Hydropathy 
Literature Information
Title High-level extracellular production of alkaline polygalacturonate lyase in Bacillus subtilis with optimized regulatory elements
DOI 10.1016/j.biortech.2013.07.129,
Publication Year 2013
PubMed ID 23973973,
Patent ID NA,
