SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB06728

Signal sequence   MGLGKKLSVAVAASFMSLSISLPGVQA

Length  27

Organism  Bacillus subtilis WB600

UniProt ID  NA,

Motif  1..27



Activity Information


Pathway  Sec

Natural/Artificial  Artificial

Subcellular location  Extracellular environment

Secretion efficiency  Alkaline polygalacturonate lyase yield: 23.3(U/ml)

Type  Sec/SPI



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB06728.cif

Structure  SPKB06728.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  2663.22934

Aliphatic index  119.2592593

Isoelectric point  10.80537956

Charge (pH=7)   1.997394232

Topological polar surface area  1007.41

Aromaticity  0.037037037

Average rotatable bonds  3.296296296

Number of hydrogen bond donors  35

Number of hydrogen bond acceptors  38

X logP energy  -10.0845

Hydropathy 



Literature Information


Title  High-level extracellular production of alkaline polygalacturonate lyase in Bacillus subtilis with optimized regulatory elements

DOI  10.1016/j.biortech.2013.07.129,

Publication Year  2013

PubMed ID  23973973,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.