General Information
SPKB ID SPKB06727
Signal sequence MKKRKRRNFKRFIAAFLVLALMISLVPA
Length 28
Organism Bacillus subtilis WB600
UniProt ID Q07833,
Motif 1..28
Activity Information
Pathway Tat
Natural/Artificial Artificial
Subcellular location Periplasmic space
Secretion efficiency Alkaline polygalacturonate lyase yield: 24.8(U/ml)
Type Tat/SPI
Structure Information
PDB ID NA,
Predicted Structure ID SPKB06727.cif
Physicochemical Information
Molecular weight 3319.21214
Aliphatic index 118.5714286
Isoelectric point 12.99206258
Charge (pH=7) 7.996790919
Topological polar surface area 1255.23
Aromaticity 0.107142857
Average rotatable bonds 4.107142857
Number of hydrogen bond donors 46
Number of hydrogen bond acceptors 41
X logP energy -5.66662
Hydropathy 
Literature Information
Title High-level extracellular production of alkaline polygalacturonate lyase in Bacillus subtilis with optimized regulatory elements
DOI 10.1016/j.biortech.2013.07.129,
Publication Year 2013
PubMed ID 23973973,
Patent ID NA,
