SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB06726

Signal sequence   MKKKRFYIRTGILLVLLAALGYTLYSAVFQNTESV

Length  35

Organism  Bacillus licheniformis MW3

UniProt ID  NA,

Motif  1..35



Activity Information


Pathway  Sec

Natural/Artificial  Artificial

Subcellular location  Extracellular environment

Secretion efficiency  Bacillus protease novo type yield: 0.22(U/ml)

Type  Sec/SPI



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB06726.cif

Structure  SPKB06726.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  4008.82124

Aliphatic index  122.5714286

Isoelectric point  10.44876237

Charge (pH=7)   3.996316455

Topological polar surface area  1539.9

Aromaticity  0.142857143

Average rotatable bonds  3.828571429

Number of hydrogen bond donors  56

Number of hydrogen bond acceptors  53

X logP energy  -9.32956

Hydropathy 



Literature Information


Title  Optimization of Protease Secretion in Bacillus subtilis and Bacillus licheniformis by Screening of Homologous and Heterologous Signal Peptides

DOI  10.1128/AEM.01146-10,

Publication Year  2010

PubMed ID  20709850,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.