SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB06716

Signal sequence   MRGKKVWISLLFALALIFTMAFGSTSSAQA

Length  30

Organism  Bacillus subtilis TEB1030

UniProt ID  NA,

Motif  1..30



Activity Information


Pathway  Sec

Natural/Artificial  Artificial

Subcellular location  Extracellular environment

Secretion efficiency  Bacillus protease novo type yield: 0.83(U/ml)

Type  Sec/SPI



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB06716.cif

Structure  SPKB06716.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  3246.91964

Aliphatic index  104.3333333

Isoelectric point  11.82305527

Charge (pH=7)   2.99739092

Topological polar surface area  1201.42

Aromaticity  0.133333333

Average rotatable bonds  3.566666667

Number of hydrogen bond donors  44

Number of hydrogen bond acceptors  43

X logP energy  -8.77173

Hydropathy 



Literature Information


Title  Optimization of Protease Secretion in Bacillus subtilis and Bacillus licheniformis by Screening of Homologous and Heterologous Signal Peptides

DOI  10.1128/AEM.01146-10,

Publication Year  2010

PubMed ID  20709850,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.