SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB06712

Signal sequence   MRMKRLRMRKHLLIAVCTLALLLSSPIVSDA

Length  31

Organism  Bacillus subtilis TEB1030

UniProt ID  NA,

Motif  1..31



Activity Information


Pathway  Sec

Natural/Artificial  Artificial

Subcellular location  Extracellular environment

Secretion efficiency  Bacillus protease novo type yield: 3.58(U/ml)

Type  Sec/SPI



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB06712.cif

Structure  SPKB06712.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  3537.45234

Aliphatic index  141.6129032

Isoelectric point  12.07925983

Charge (pH=7)   5.026761846

Topological polar surface area  1374.07

Aromaticity  0

Average rotatable bonds  3.935483871

Number of hydrogen bond donors  52

Number of hydrogen bond acceptors  48

X logP energy  -9.46652

Hydropathy 



Literature Information


Title  Optimization of Protease Secretion in Bacillus subtilis and Bacillus licheniformis by Screening of Homologous and Heterologous Signal Peptides

DOI  10.1128/AEM.01146-10,

Publication Year  2010

PubMed ID  20709850,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.