General Information
SPKB ID SPKB06600
Signal sequence MKKVMLATALFLGLTPAGANA
Length 21
Organism Bacillus subtilis WB600
UniProt ID P39116,
Motif 1..21
Activity Information
Pathway Sec
Natural/Artificial Artificial
Subcellular location Extracellular environment
Secretion efficiency Aminopeptidase yield: 1.93(U/ml)
Type Sec/SPI
Structure Information
PDB ID 1BN8,
Predicted Structure ID SPKB06600.cif
Physicochemical Information
Molecular weight 2118.62394
Aliphatic index 111.9047619
Isoelectric point 10.80537956
Charge (pH=7) 1.997394232
Topological polar surface area 772.12
Aromaticity 0.047619048
Average rotatable bonds 3.285714286
Number of hydrogen bond donors 26
Number of hydrogen bond acceptors 29
X logP energy -5.5529
Hydropathy 
Literature Information
Title Construction of a highly active secretory expression system via an engineered dual promoter and a highly efficient signal peptide in Bacillus subtilis
DOI 10.1016/j.nbt.2016.01.005,
Publication Year 2016
PubMed ID 26820123,
Patent ID NA,
