General Information
SPKB ID SPKB06599
Signal sequence MRIFKKAVFVIMISFLIATVNVNTAHA
Length 27
Organism Bacillus subtilis WB600
UniProt ID P35150,
Motif 1..27
Activity Information
Pathway Sec
Natural/Artificial Artificial
Subcellular location Extracellular environment
Secretion efficiency Aminopeptidase yield: 2.45(U/ml)
Type Sec/SPI
Structure Information
PDB ID NA,
Predicted Structure ID SPKB06599.cif
Physicochemical Information
Molecular weight 3035.74564
Aliphatic index 130
Isoelectric point 11.82306209
Charge (pH=7) 3.088300011
Topological polar surface area 1109.41
Aromaticity 0.111111111
Average rotatable bonds 3.703703704
Number of hydrogen bond donors 39
Number of hydrogen bond acceptors 39
X logP energy -5.83673
Hydropathy 
Literature Information
Title Construction of a highly active secretory expression system via an engineered dual promoter and a highly efficient signal peptide in Bacillus subtilis
DOI 10.1016/j.nbt.2016.01.005,
Publication Year 2016
PubMed ID 26820123,
Patent ID NA,
