General Information
SPKB ID SPKB06598
Signal sequence MKLKTKASIKFGICVGLLCLSITGFTPFFNSTHAEA
Length 36
Organism Bacillus subtilis WB600
UniProt ID P39824,
Motif 1..36
Activity Information
Pathway Sec
Natural/Artificial Artificial
Subcellular location Extracellular environment
Secretion efficiency Aminopeptidase yield: 2.6(U/ml)
Type Sec/SPI
Structure Information
PDB ID NA,
Predicted Structure ID SPKB06598.cif
Physicochemical Information
Molecular weight 3875.66084
Aliphatic index 92.22222222
Isoelectric point 9.601448848
Charge (pH=7) 2.965538946
Topological polar surface area 1427.79
Aromaticity 0.111111111
Average rotatable bonds 3.555555556
Number of hydrogen bond donors 52
Number of hydrogen bond acceptors 54
X logP energy -11.8893
Hydropathy 
Literature Information
Title Construction of a highly active secretory expression system via an engineered dual promoter and a highly efficient signal peptide in Bacillus subtilis
DOI 10.1016/j.nbt.2016.01.005,
Publication Year 2016
PubMed ID 26820123,
Patent ID NA,
