General Information
SPKB ID SPKB06595
Signal sequence MKKGIIRFLLVSFVLFFALSTGITGVQA
Length 28
Organism Bacillus subtilis WB600
UniProt ID P29141,
Motif 1..28
Activity Information
Pathway Sec
Natural/Artificial Artificial
Subcellular location Extracellular environment
Secretion efficiency Aminopeptidase yield: 4.45(U/ml)
Type Sec/SPI
Structure Information
PDB ID NA,
Predicted Structure ID SPKB06595.cif
Physicochemical Information
Molecular weight 3057.77414
Aliphatic index 135.7142857
Isoelectric point 11.82305527
Charge (pH=7) 2.99739092
Topological polar surface area 1086.97
Aromaticity 0.142857143
Average rotatable bonds 3.642857143
Number of hydrogen bond donors 39
Number of hydrogen bond acceptors 38
X logP energy -5.03053
Hydropathy 
Literature Information
Title Construction of a highly active secretory expression system via an engineered dual promoter and a highly efficient signal peptide in Bacillus subtilis
DOI 10.1016/j.nbt.2016.01.005,
Publication Year 2016
PubMed ID 26820123,
Patent ID NA,
