General Information
SPKB ID SPKB06594
Signal sequence VGLGKKLSVRVAASFMSLSISLPGVQA
Length 27
Organism Bacillus subtilis WB600
UniProt ID NA,
Motif 1..27
Activity Information
Pathway Sec
Natural/Artificial Artificial
Subcellular location Extracellular environment
Secretion efficiency Aminopeptidase yield: 4.82(U/ml)
Type Sec/SPI
Structure Information
PDB ID NA,
Predicted Structure ID SPKB06594.cif
Physicochemical Information
Molecular weight 2716.27804
Aliphatic index 126.2962963
Isoelectric point 11.82305527
Charge (pH=7) 2.99739092
Topological polar surface area 1069.31
Aromaticity 0.037037037
Average rotatable bonds 3.37037037
Number of hydrogen bond donors 38
Number of hydrogen bond acceptors 38
X logP energy -10.93823
Hydropathy 
Literature Information
Title Construction of a highly active secretory expression system via an engineered dual promoter and a highly efficient signal peptide in Bacillus subtilis
DOI 10.1016/j.nbt.2016.01.005,
Publication Year 2016
PubMed ID 26820123,
Patent ID NA,
