General Information
SPKB ID SPKB06592
Signal sequence MFAKRFKTSLLPLFAGFLLLFHLVLAG
Length 27
Organism Bacillus subtilis WB600
UniProt ID P00691,
Motif 1..27
Activity Information
Pathway Sec
Natural/Artificial Artificial
Subcellular location Extracellular environment
Secretion efficiency Aminopeptidase yield: 5.12(U/ml)
Type Sec/SPI
Structure Information
PDB ID NA,
Predicted Structure ID SPKB06592.cif
Physicochemical Information
Molecular weight 3051.81564
Aliphatic index 137.4074074
Isoelectric point 11.82306209
Charge (pH=7) 3.088300011
Topological polar surface area 994.21
Aromaticity 0.185185185
Average rotatable bonds 3.62962963
Number of hydrogen bond donors 35
Number of hydrogen bond acceptors 35
X logP energy -0.31663
Hydropathy 
Literature Information
Title Construction of a highly active secretory expression system via an engineered dual promoter and a highly efficient signal peptide in Bacillus subtilis
DOI 10.1016/j.nbt.2016.01.005,
Publication Year 2016
PubMed ID 26820123,
Patent ID NA,
