General Information
SPKB ID SPKB06591
Signal sequence MNIKKFAKQATVLTFTTALLAGGATQAFA
Length 29
Organism Bacillus subtilis WB600
UniProt ID P05655,
Motif 1..29
Activity Information
Pathway Sec
Natural/Artificial Artificial
Subcellular location Extracellular environment
Secretion efficiency Aminopeptidase yield: 5.12(U/ml)
Type Sec/SPI
Structure Information
PDB ID 3BYJ,
Predicted Structure ID SPKB06591.cif
Physicochemical Information
Molecular weight 3014.57624
Aliphatic index 87.93103448
Isoelectric point 11.10307074
Charge (pH=7) 2.997099198
Topological polar surface area 1186.6
Aromaticity 0.103448276
Average rotatable bonds 3.448275862
Number of hydrogen bond donors 41
Number of hydrogen bond acceptors 42
X logP energy -11.6062
Hydropathy 
Literature Information
Title Construction of a highly active secretory expression system via an engineered dual promoter and a highly efficient signal peptide in Bacillus subtilis
DOI 10.1016/j.nbt.2016.01.005,
Publication Year 2016
PubMed ID 26820123,
Patent ID NA,
