General Information
SPKB ID SPKB06590
Signal sequence MKKIGLLFMLCLAALFTIGFPAQQADA
Length 27
Organism Bacillus subtilis WB600
UniProt ID O31737,
Motif 1..27
Activity Information
Pathway Sec
Natural/Artificial Artificial
Subcellular location Extracellular environment
Secretion efficiency Aminopeptidase yield: 5.19(U/ml)
Type Sec/SPI
Structure Information
PDB ID NA,
Predicted Structure ID SPKB06590.cif
Physicochemical Information
Molecular weight 2912.60264
Aliphatic index 119.6296296
Isoelectric point 8.540492971
Charge (pH=7) 0.935866
Topological polar surface area 1006.88
Aromaticity 0.111111111
Average rotatable bonds 3.555555556
Number of hydrogen bond donors 34
Number of hydrogen bond acceptors 37
X logP energy -3.9971
Hydropathy 
Literature Information
Title Construction of a highly active secretory expression system via an engineered dual promoter and a highly efficient signal peptide in Bacillus subtilis
DOI 10.1016/j.nbt.2016.01.005,
Publication Year 2016
PubMed ID 26820123,
Patent ID NA,
