General Information
SPKB ID SPKB06589
Signal sequence MKKMLMLAFTFLLALTIHVGEASA
Length 24
Organism Bacillus subtilis WB600
UniProt ID O34541,
Motif 1..24
Activity Information
Pathway Sec
Natural/Artificial Artificial
Subcellular location Extracellular environment
Secretion efficiency Aminopeptidase yield: 6.23(U/ml)
Type Sec/SPI
Structure Information
PDB ID NA,
Predicted Structure ID SPKB06589.cif
Physicochemical Information
Molecular weight 2637.29554
Aliphatic index 126.25
Isoelectric point 9.539374794
Charge (pH=7) 1.090078445
Topological polar surface area 911.33
Aromaticity 0.083333333
Average rotatable bonds 3.708333333
Number of hydrogen bond donors 32
Number of hydrogen bond acceptors 35
X logP energy -3.2205
Hydropathy 
Literature Information
Title Construction of a highly active secretory expression system via an engineered dual promoter and a highly efficient signal peptide in Bacillus subtilis
DOI 10.1016/j.nbt.2016.01.005,
Publication Year 2016
PubMed ID 26820123,
Patent ID NA,
