SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB06587

Signal sequence   MRKKTKNRLISSVLSTVVISSLLFPGAAGA

Length  30

Organism  Bacillus subtilis WB600

UniProt ID  NA,

Motif  1..30



Activity Information


Pathway  Sec

Natural/Artificial  Artificial

Subcellular location  Extracellular environment

Secretion efficiency  Aminopeptidase yield: 16.62(U/ml)

Type  Sec/SPI



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB06587.cif

Structure  SPKB06587.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  3145.79784

Aliphatic index  117

Isoelectric point  12.54607567

Charge (pH=7)   4.997092575

Topological polar surface area  1284.99

Aromaticity  0.033333333

Average rotatable bonds  3.566666667

Number of hydrogen bond donors  47

Number of hydrogen bond acceptors  45

X logP energy  -13.96136

Hydropathy 



Literature Information


Title  Construction of a highly active secretory expression system via an engineered dual promoter and a highly efficient signal peptide in Bacillus subtilis

DOI  10.1016/j.nbt.2016.01.005,

Publication Year  2016

PubMed ID  26820123,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.