Peptide-Information

General Information

SPKB ID SPKB06586

Signal sequence MKKQIITATTAVVLGSTLFAGAASA

Length 25

Organism Bacillus subtilis WB600

UniProt ID P54421,

Motif 1..25

Activity Information

Pathway Sec

Natural/Artificial Artificial

Subcellular location Extracellular environment

Secretion efficiency Aminopeptidase yield: 24.19(U/ml)

Type Sec/SPI

Structure Information

PDB ID NA,

Predicted Structure ID SPKB06586.cif

Structure SPKB06586.cif

Very high (pLDDT > 90)

Confident (90 > pLDDT > 70)

Low (70 > pLDDT > 50)

Very low (pLDDT < 50)

Loading PAE…

Expected Position Error (Ångströms)

Physicochemical Information

Molecular weight 2450.91954

Aliphatic index 109.6

Isoelectric point 10.80537956

Charge (pH=7) 1.997394232

Topological polar surface area 978.23

Aromaticity 0.04

Average rotatable bonds 3.24

Number of hydrogen bond donors 35

Number of hydrogen bond acceptors 36

X logP energy -11.0608

Hydropathy

Literature Information

Title Construction of a highly active secretory expression system via an engineered dual promoter and a highly efficient signal peptide in Bacillus subtilis

DOI 10.1016/j.nbt.2016.01.005,

Publication Year 2016

PubMed ID 26820123,

Patent ID NA,