General Information
SPKB ID SPKB06584
Signal sequence MKISMQKADFWKKAAISLLVFTMFFTLMMSETVFA
Length 35
Organism Bacillus subtilis WB600
UniProt ID O07921,
Motif 1..35
Activity Information
Pathway Sec
Natural/Artificial Artificial
Subcellular location Extracellular environment
Secretion efficiency Aminopeptidase yield: 30.49(U/ml)
Type Sec/SPI
Structure Information
PDB ID NA,
Predicted Structure ID SPKB06584.cif
Physicochemical Information
Molecular weight 4094.03294
Aliphatic index 83.71428571
Isoelectric point 10.333323
Charge (pH=7) 1.999025771
Topological polar surface area 1411.66
Aromaticity 0.171428571
Average rotatable bonds 3.942857143
Number of hydrogen bond donors 50
Number of hydrogen bond acceptors 54
X logP energy -5.5531
Hydropathy 
Literature Information
Title Construction of a highly active secretory expression system via an engineered dual promoter and a highly efficient signal peptide in Bacillus subtilis
DOI 10.1016/j.nbt.2016.01.005,
Publication Year 2016
PubMed ID 26820123,
Patent ID NA,
