General Information
SPKB ID SPKB06583
Signal sequence MKKLLTVMTMAVLTAGTLLLPAQS
Length 24
Organism Bacillus subtilis WB600
UniProt ID P25152,
Motif 1..24
Activity Information
Pathway Sec
Natural/Artificial Artificial
Subcellular location Extracellular environment
Secretion efficiency Aminopeptidase yield: 73.6(U/ml)
Type Sec/SPI
Structure Information
PDB ID 6HC6,
Predicted Structure ID SPKB06583.cif
Physicochemical Information
Molecular weight 2532.19714
Aliphatic index 134.1666667
Isoelectric point 10.80537956
Charge (pH=7) 1.997394232
Topological polar surface area 920.11
Aromaticity 0
Average rotatable bonds 3.541666667
Number of hydrogen bond donors 32
Number of hydrogen bond acceptors 36
X logP energy -6.3671
Hydropathy 
Literature Information
Title Construction of a highly active secretory expression system via an engineered dual promoter and a highly efficient signal peptide in Bacillus subtilis
DOI 10.1016/j.nbt.2016.01.005,
Publication Year 2016
PubMed ID 26820123,
Patent ID NA,
