SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB06570

Signal sequence   MKKKRKGCFAAAGFMMIFVFVIA

Length  23

Organism  Bacillus subtilis TEB1030

UniProt ID  NA,

Motif  1..23



Activity Information


Pathway  Sec

Natural/Artificial  Artificial

Subcellular location  Extracellular environment

Secretion efficiency  NA

Type  Sec/SPI



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB06570.cif

Structure  SPKB06570.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  2595.32514

Aliphatic index  76.52173913

Isoelectric point  11.24912534

Charge (pH=7)   4.934826137

Topological polar surface area  869.5

Aromaticity  0.173913043

Average rotatable bonds  3.869565217

Number of hydrogen bond donors  32

Number of hydrogen bond acceptors  33

X logP energy  -2.25033

Hydropathy 



Literature Information


Title  Systematic screening of all signal peptides from Bacillus subtilis: A powerful strategy in optimizing heterologous protein secretion in gram-positive bacteria

DOI  10.1016/j.jmb.2006.07.034,

Publication Year  2006

PubMed ID  16930615,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.