SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB06546

Signal sequence   MKKKQVSHAIIISVMLSFVIAVFHTIHA

Length  28

Organism  Bacillus subtilis TEB1030

UniProt ID  NA,

Motif  1..28



Activity Information


Pathway  Sec

Natural/Artificial  Artificial

Subcellular location  Extracellular environment

Secretion efficiency  NA

Type  Sec/SPI



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB06546.cif

Structure  SPKB06546.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  3149.89284

Aliphatic index  135.7142857

Isoelectric point  11.10311933

Charge (pH=7)   3.269826471

Topological polar surface area  1137.13

Aromaticity  0.071428571

Average rotatable bonds  3.785714286

Number of hydrogen bond donors  40

Number of hydrogen bond acceptors  42

X logP energy  -5.6526

Hydropathy 



Literature Information


Title  Systematic screening of all signal peptides from Bacillus subtilis: A powerful strategy in optimizing heterologous protein secretion in gram-positive bacteria

DOI  10.1016/j.jmb.2006.07.034,

Publication Year  2006

PubMed ID  16930615,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.