SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB06521

Signal sequence   MKVCQKSIVRFLVSLIIGTFVISVPFMANA

Length  30

Organism  Bacillus subtilis TEB1030

UniProt ID  O35015,

Motif  1..30



Activity Information


Pathway  Sec

Natural/Artificial  Artificial

Subcellular location  Extracellular environment

Secretion efficiency  NA

Type  Sec/SPI



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB06521.cif

Structure  SPKB06521.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  3313.12454

Aliphatic index  133

Isoelectric point  10.79319833

Charge (pH=7)   2.935416204

Topological polar surface area  1179.47

Aromaticity  0.1

Average rotatable bonds  3.633333333

Number of hydrogen bond donors  42

Number of hydrogen bond acceptors  43

X logP energy  -6.62453

Hydropathy 



Literature Information


Title  Systematic screening of all signal peptides from Bacillus subtilis: A powerful strategy in optimizing heterologous protein secretion in gram-positive bacteria

DOI  10.1016/j.jmb.2006.07.034,

Publication Year  2006

PubMed ID  16930615,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.