SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB06519

Signal sequence   MLRKKHFSWMLVILILIAVLSFIKLPYYITKPGEA

Length  35

Organism  Bacillus subtilis TEB1030

UniProt ID  NA,

Motif  1..35



Activity Information


Pathway  Sec

Natural/Artificial  Artificial

Subcellular location  Extracellular environment

Secretion efficiency  NA

Type  Sec/SPI



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB06519.cif

Structure  SPKB06519.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  4134.18234

Aliphatic index  144.8571429

Isoelectric point  10.57742586

Charge (pH=7)   4.087784238

Topological polar surface area  1384.04

Aromaticity  0.142857143

Average rotatable bonds  3.857142857

Number of hydrogen bond donors  49

Number of hydrogen bond acceptors  50

X logP energy  -0.02833

Hydropathy 



Literature Information


Title  Systematic screening of all signal peptides from Bacillus subtilis: A powerful strategy in optimizing heterologous protein secretion in gram-positive bacteria

DOI  10.1016/j.jmb.2006.07.034,

Publication Year  2006

PubMed ID  16930615,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.