SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB06517

Signal sequence   MKKVWIGIGIAVIVALFVGINIYRSAAPTSGSA

Length  33

Organism  Bacillus subtilis TEB1030

UniProt ID  NA,

Motif  1..33



Activity Information


Pathway  Sec

Natural/Artificial  Artificial

Subcellular location  Extracellular environment

Secretion efficiency  Cutinase yield: 0.01(U/ml)

Type  Sec/SPI



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB06517.cif

Structure  SPKB06517.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  3404.11734

Aliphatic index  133.030303

Isoelectric point  10.90180955

Charge (pH=7)   2.996540506

Topological polar surface area  1259.7

Aromaticity  0.090909091

Average rotatable bonds  3.303030303

Number of hydrogen bond donors  45

Number of hydrogen bond acceptors  44

X logP energy  -8.58763

Hydropathy 



Literature Information


Title  Systematic screening of all signal peptides from Bacillus subtilis: A powerful strategy in optimizing heterologous protein secretion in gram-positive bacteria

DOI  10.1016/j.jmb.2006.07.034,

Publication Year  2006

PubMed ID  16930615,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.