SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB06513

Signal sequence   MLSVEMISRQNRCHYVYKGGNMMRRILHIVLITALMFLNVMYTFEA

Length  46

Organism  Bacillus subtilis TEB1030

UniProt ID  P54602,

Motif  1..46



Activity Information


Pathway  Sec

Natural/Artificial  Artificial

Subcellular location  Extracellular environment

Secretion efficiency  Cutinase yield: 0.04(U/ml)

Type  Sec/SPI



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB06513.cif

Structure  SPKB06513.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  5495.66324

Aliphatic index  105.8695652

Isoelectric point  9.803847793

Charge (pH=7)   3.118518489

Topological polar surface area  2092.37

Aromaticity  0.108695652

Average rotatable bonds  4.02173913

Number of hydrogen bond donors  76

Number of hydrogen bond acceptors  74

X logP energy  -13.18432

Hydropathy 



Literature Information


Title  Systematic screening of all signal peptides from Bacillus subtilis: A powerful strategy in optimizing heterologous protein secretion in gram-positive bacteria

DOI  10.1016/j.jmb.2006.07.034,

Publication Year  2006

PubMed ID  16930615,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.