SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB06508

Signal sequence   MARKKKKKHEDEHVDESWLVPYADILTLLLALFIVLYASS

Length  40

Organism  Bacillus subtilis TEB1030

UniProt ID  NA,

Motif  1..40



Activity Information


Pathway  Sec

Natural/Artificial  Artificial

Subcellular location  Extracellular environment

Secretion efficiency  Cutinase yield: 0.07(U/ml)

Type  Sec/SPI



Structure Information


PDB ID  6YSL,

Predicted Structure ID  SPKB06508.cif

Structure  SPKB06508.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  4672.50614

Aliphatic index  119.5

Isoelectric point  7.692794607

Charge (pH=7)   0.183287993

Topological polar surface area  1799.76

Aromaticity  0.1

Average rotatable bonds  3.925

Number of hydrogen bond donors  63

Number of hydrogen bond acceptors  62

X logP energy  -8.89333

Hydropathy 



Literature Information


Title  Systematic screening of all signal peptides from Bacillus subtilis: A powerful strategy in optimizing heterologous protein secretion in gram-positive bacteria

DOI  10.1016/j.jmb.2006.07.034,

Publication Year  2006

PubMed ID  16930615,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.