SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB06499

Signal sequence   MKRRKFSSVVAAVLIFALIFSLFSPGTKAAA

Length  31

Organism  Bacillus subtilis TEB1030

UniProt ID  P54423,

Motif  1..31



Activity Information


Pathway  Sec

Natural/Artificial  Artificial

Subcellular location  Periplasmic space

Secretion efficiency  Cutinase yield: 0.12(U/ml)

Type  Sec/SPI



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB06499.cif

Structure  SPKB06499.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  3328.06564

Aliphatic index  110.3225806

Isoelectric point  12.54607567

Charge (pH=7)   4.997092575

Topological polar surface area  1230.54

Aromaticity  0.129032258

Average rotatable bonds  3.516129032

Number of hydrogen bond donors  45

Number of hydrogen bond acceptors  43

X logP energy  -8.61476

Hydropathy 



Literature Information


Title  Systematic screening of all signal peptides from Bacillus subtilis: A powerful strategy in optimizing heterologous protein secretion in gram-positive bacteria

DOI  10.1016/j.jmb.2006.07.034,

Publication Year  2006

PubMed ID  16930615,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.