SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB06490

Signal sequence   MNTLANWKKFLLVAVIICFLVPIMTKAEIAEA

Length  32

Organism  Bacillus subtilis TEB1030

UniProt ID  P96740,

Motif  1..32



Activity Information


Pathway  Sec

Natural/Artificial  Artificial

Subcellular location  Extracellular environment

Secretion efficiency  Cutinase yield: 0.25(U/ml)

Type  Sec/SPI



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB06490.cif

Structure  SPKB06490.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  3592.46044

Aliphatic index  140.3125

Isoelectric point  8.535917

Charge (pH=7)   0.938674727

Topological polar surface area  1251.72

Aromaticity  0.09375

Average rotatable bonds  3.6875

Number of hydrogen bond donors  43

Number of hydrogen bond acceptors  45

X logP energy  -3.356

Hydropathy 



Literature Information


Title  Systematic screening of all signal peptides from Bacillus subtilis: A powerful strategy in optimizing heterologous protein secretion in gram-positive bacteria

DOI  10.1016/j.jmb.2006.07.034,

Publication Year  2006

PubMed ID  16930615,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.