SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB06489

Signal sequence   MKKLLAAGIIGLLTVSIASPSFA

Length  23

Organism  Bacillus subtilis TEB1030

UniProt ID  P70961,

Motif  1..23



Activity Information


Pathway  Sec

Natural/Artificial  Artificial

Subcellular location  Extracellular environment

Secretion efficiency  Cutinase yield: 0.26(U/ml)

Type  Sec/SPI



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB06489.cif

Structure  SPKB06489.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  2301.85644

Aliphatic index  148.6956522

Isoelectric point  10.80537956

Charge (pH=7)   1.997394232

Topological polar surface area  827.69

Aromaticity  0.043478261

Average rotatable bonds  3.304347826

Number of hydrogen bond donors  29

Number of hydrogen bond acceptors  31

X logP energy  -5.4973

Hydropathy 



Literature Information


Title  Systematic screening of all signal peptides from Bacillus subtilis: A powerful strategy in optimizing heterologous protein secretion in gram-positive bacteria

DOI  10.1016/j.jmb.2006.07.034,

Publication Year  2006

PubMed ID  16930615,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.