General Information
SPKB ID SPKB06475
Signal sequence MKKRLIGFLVLVPALIMSGITLIEA
Length 25
Organism Bacillus subtilis TEB1030
UniProt ID O31992,
Motif 1..25
Activity Information
Pathway Sec
Natural/Artificial Artificial
Subcellular location Extracellular environment
Secretion efficiency Cutinase yield: 0.58(U/ml)
Type Sec/SPI
Structure Information
PDB ID NA,
Predicted Structure ID SPKB06475.cif
Physicochemical Information
Molecular weight 2727.48944
Aliphatic index 171.6
Isoelectric point 10.7890093
Charge (pH=7) 1.999166043
Topological polar surface area 944.63
Aromaticity 0.04
Average rotatable bonds 3.72
Number of hydrogen bond donors 33
Number of hydrogen bond acceptors 34
X logP energy -1.92273
Hydropathy 
Literature Information
Title Systematic screening of all signal peptides from Bacillus subtilis: A powerful strategy in optimizing heterologous protein secretion in gram-positive bacteria
DOI 10.1016/j.jmb.2006.07.034,
Publication Year 2006
PubMed ID 16930615,
Patent ID NA,
