General Information
SPKB ID SPKB06462
Signal sequence MKKWLIIAVSLAIAIVLFMYTKGEAKA
Length 27
Organism Bacillus subtilis TEB1030
UniProt ID O32258,
Motif 1..27
Activity Information
Pathway Sec
Natural/Artificial Artificial
Subcellular location Extracellular environment
Secretion efficiency Cutinase yield: 1.09(U/ml)
Type Sec/SPI
Structure Information
PDB ID NA,
Predicted Structure ID SPKB06462.cif
Physicochemical Information
Molecular weight 3009.78834
Aliphatic index 141.1111111
Isoelectric point 10.4849221
Charge (pH=7) 2.997728873
Topological polar surface area 1037.78
Aromaticity 0.111111111
Average rotatable bonds 3.777777778
Number of hydrogen bond donors 37
Number of hydrogen bond acceptors 38
X logP energy -1.4562
Hydropathy 
Literature Information
Title Systematic screening of all signal peptides from Bacillus subtilis: A powerful strategy in optimizing heterologous protein secretion in gram-positive bacteria
DOI 10.1016/j.jmb.2006.07.034,
Publication Year 2006
PubMed ID 16930615,
Patent ID NA,
