SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB06460

Signal sequence   MKQFAITLSVLCALILLVPTLLVIPFQHNKEAGA

Length  34

Organism  Bacillus subtilis TEB1030

UniProt ID  NA,

Motif  1..34



Activity Information


Pathway  Sec

Natural/Artificial  Artificial

Subcellular location  Extracellular environment

Secretion efficiency  Cutinase yield: 1.28(U/ml)

Type  Sec/SPI



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB06460.cif

Structure  SPKB06460.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  3693.55054

Aliphatic index  152.0588235

Isoelectric point  8.548069757

Charge (pH=7)   1.028103729

Topological polar surface area  1314.02

Aromaticity  0.058823529

Average rotatable bonds  3.529411765

Number of hydrogen bond donors  44

Number of hydrogen bond acceptors  47

X logP energy  -6.0337

Hydropathy 



Literature Information


Title  Systematic screening of all signal peptides from Bacillus subtilis: A powerful strategy in optimizing heterologous protein secretion in gram-positive bacteria

DOI  10.1016/j.jmb.2006.07.034,

Publication Year  2006

PubMed ID  16930615,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.