General Information
SPKB ID SPKB06452
Signal sequence MKKWIYVVLVLSIAGIGGFSVHA
Length 23
Organism Bacillus subtilis TEB1030
UniProt ID P54427,
Motif 1..23
Activity Information
Pathway Sec
Natural/Artificial Artificial
Subcellular location Extracellular environment
Secretion efficiency Cutinase yield: 1.44(U/ml)
Type Sec/SPI
Structure Information
PDB ID 5E2F,
Predicted Structure ID SPKB06452.cif
Physicochemical Information
Molecular weight 2489.06004
Aliphatic index 143.9130435
Isoelectric point 10.24773591
Charge (pH=7) 2.087452908
Topological polar surface area 860.72
Aromaticity 0.130434783
Average rotatable bonds 3.47826087
Number of hydrogen bond donors 31
Number of hydrogen bond acceptors 31
X logP energy -2.2711
Hydropathy 
Literature Information
Title Systematic screening of all signal peptides from Bacillus subtilis: A powerful strategy in optimizing heterologous protein secretion in gram-positive bacteria
DOI 10.1016/j.jmb.2006.07.034,
Publication Year 2006
PubMed ID 16930615,
Patent ID NA,
