General Information
SPKB ID SPKB06451
Signal sequence MKKSIKLYVAVLLLFVVASVPYMHQAALA
Length 29
Organism Bacillus subtilis TEB1030
UniProt ID P39844,
Motif 1..29
Activity Information
Pathway Sec
Natural/Artificial Artificial
Subcellular location Extracellular environment
Secretion efficiency Cutinase yield: 1.46(U/ml)
Type Sec/SPI
Structure Information
PDB ID NA,
Predicted Structure ID SPKB06451.cif
Physicochemical Information
Molecular weight 3205.00964
Aliphatic index 147.9310345
Isoelectric point 10.33016113
Charge (pH=7) 3.08630746
Topological polar surface area 1100.08
Aromaticity 0.103448276
Average rotatable bonds 3.586206897
Number of hydrogen bond donors 38
Number of hydrogen bond acceptors 41
X logP energy -2.6166
Hydropathy 
Literature Information
Title Systematic screening of all signal peptides from Bacillus subtilis: A powerful strategy in optimizing heterologous protein secretion in gram-positive bacteria
DOI 10.1016/j.jmb.2006.07.034,
Publication Year 2006
PubMed ID 16930615,
Patent ID NA,
