General Information
SPKB ID SPKB06449
Signal sequence MNKKYFVLIVCIIFTSALFPTFSSVTA
Length 27
Organism Bacillus subtilis TEB1030
UniProt ID O32041,
Motif 1..27
Activity Information
Pathway Sec
Natural/Artificial Artificial
Subcellular location Extracellular environment
Secretion efficiency Cutinase yield: 1.49(U/ml)
Type Sec/SPI
Structure Information
PDB ID 9A7D,
Predicted Structure ID SPKB06449.cif
Physicochemical Information
Molecular weight 3041.70004
Aliphatic index 111.8518519
Isoelectric point 9.405774727
Charge (pH=7) 1.934569102
Topological polar surface area 1047.87
Aromaticity 0.185185185
Average rotatable bonds 3.518518519
Number of hydrogen bond donors 38
Number of hydrogen bond acceptors 40
X logP energy -5.3977
Hydropathy 
Literature Information
Title Systematic screening of all signal peptides from Bacillus subtilis: A powerful strategy in optimizing heterologous protein secretion in gram-positive bacteria
DOI 10.1016/j.jmb.2006.07.034,
Publication Year 2006
PubMed ID 16930615,
Patent ID NA,
