General Information
SPKB ID SPKB06448
Signal sequence MNIKKFAKQATVLTFTTALLAGGATQAFA
Length 29
Organism Bacillus subtilis TEB1030
UniProt ID P05655,
Motif 1..29
Activity Information
Pathway Sec
Natural/Artificial Artificial
Subcellular location Extracellular environment
Secretion efficiency Cutinase yield: 1.51(U/ml)
Type Sec/SPI
Structure Information
PDB ID 3BYJ,
Predicted Structure ID SPKB06448.cif
Physicochemical Information
Molecular weight 3014.57624
Aliphatic index 87.93103448
Isoelectric point 11.10307074
Charge (pH=7) 2.997099198
Topological polar surface area 1186.6
Aromaticity 0.103448276
Average rotatable bonds 3.448275862
Number of hydrogen bond donors 41
Number of hydrogen bond acceptors 42
X logP energy -11.6062
Hydropathy 
Literature Information
Title Systematic screening of all signal peptides from Bacillus subtilis: A powerful strategy in optimizing heterologous protein secretion in gram-positive bacteria
DOI 10.1016/j.jmb.2006.07.034,
Publication Year 2006
PubMed ID 16930615,
Patent ID NA,
