SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB06435

Signal sequence   MKKRLIQVMIMFTLLLTMAFSADA

Length  24

Organism  Bacillus subtilis TEB1030

UniProt ID  P05656,

Motif  1..24



Activity Information


Pathway  Sec

Natural/Artificial  Artificial

Subcellular location  Extracellular environment

Secretion efficiency  Cutinase yield: 1.95(U/ml)

Type  Sec/SPI



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB06435.cif

Structure  SPKB06435.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  2773.50764

Aliphatic index  122.0833333

Isoelectric point  10.78900924

Charge (pH=7)   1.997837405

Topological polar surface area  987.64

Aromaticity  0.083333333

Average rotatable bonds  4

Number of hydrogen bond donors  35

Number of hydrogen bond acceptors  37

X logP energy  -3.94583

Hydropathy 



Literature Information


Title  Systematic screening of all signal peptides from Bacillus subtilis: A powerful strategy in optimizing heterologous protein secretion in gram-positive bacteria

DOI  10.1016/j.jmb.2006.07.034,

Publication Year  2006

PubMed ID  16930615,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.