SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB06432

Signal sequence   MKTKTLFIFSAILTLSIFAPNETFA

Length  25

Organism  Bacillus subtilis TEB1030

UniProt ID  O05213,

Motif  1..25



Activity Information


Pathway  Sec

Natural/Artificial  Artificial

Subcellular location  Extracellular environment

Secretion efficiency  Cutinase yield: 2.09(U/ml)

Type  Sec/SPI



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB06432.cif

Structure  SPKB06432.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  2805.36804

Aliphatic index  105.6

Isoelectric point  9.537300927

Charge (pH=7)   0.999169354

Topological polar surface area  1006.74

Aromaticity  0.16

Average rotatable bonds  3.6

Number of hydrogen bond donors  35

Number of hydrogen bond acceptors  37

X logP energy  -5.8941

Hydropathy 



Literature Information


Title  Systematic screening of all signal peptides from Bacillus subtilis: A powerful strategy in optimizing heterologous protein secretion in gram-positive bacteria

DOI  10.1016/j.jmb.2006.07.034,

Publication Year  2006

PubMed ID  16930615,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.