SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB06416

Signal sequence   MKKRKRRNFKRFIAAFLVLALMISLVPADVLA

Length  32

Organism  Bacillus subtilis TEB1030

UniProt ID  H9TZU6,

Motif  1..32



Activity Information


Pathway  Tat

Natural/Artificial  Artificial

Subcellular location  Periplasmic space

Secretion efficiency  Cutinase yield: 2.88(U/ml)

Type  Tat/SPI



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB06416.cif

Structure  SPKB06416.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  3717.67154

Aliphatic index  128.125

Isoelectric point  12.53169954

Charge (pH=7)   6.997237403

Topological polar surface area  1408.93

Aromaticity  0.09375

Average rotatable bonds  4

Number of hydrogen bond donors  51

Number of hydrogen bond acceptors  46

X logP energy  -6.53072

Hydropathy 



Literature Information


Title  Systematic screening of all signal peptides from Bacillus subtilis: A powerful strategy in optimizing heterologous protein secretion in gram-positive bacteria

DOI  10.1016/j.jmb.2006.07.034,

Publication Year  2006

PubMed ID  16930615,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.