SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB06395

Signal sequence   MKKAFILSAAAASVGLFTFGGVQQASA

Length  27

Organism  Bacillus subtilis

UniProt ID  NA,

Motif  1..27



Activity Information


Pathway  NA

Natural/Artificial  Artificial

Subcellular location  Extracellular environment

Secretion efficiency  NA

Type  NA



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB06395.cif

Structure  SPKB06395.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  2672.13764

Aliphatic index  90.74074074

Isoelectric point  10.80537956

Charge (pH=7)   1.997394232

Topological polar surface area  1039.06

Aromaticity  0.111111111

Average rotatable bonds  3.259259259

Number of hydrogen bond donors  36

Number of hydrogen bond acceptors  37

X logP energy  -10.8852

Hydropathy 



Literature Information


Title  Increased Expression Levels of Thermophilic Serine Protease TTHA0724 through Signal Peptide Screening in Bacillus subtilis and Applications of the Enzyme

DOI  10.3390/ijms242115950,

Publication Year  2023

PubMed ID  37958933,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.