SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB06324

Signal sequence   MKRRKFSSVVAAVLIFALIFSLFSPGTKAAA

Length  31

Organism  Bacillus subtilis

UniProt ID  P54423,

Motif  1..31



Activity Information


Pathway  Sec

Natural/Artificial  Artificial

Subcellular location  Extracellular environment

Secretion efficiency  NA

Type  Sec/SPI



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB06324.cif

Structure  SPKB06324.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  3328.06564

Aliphatic index  110.3225806

Isoelectric point  12.54607567

Charge (pH=7)   4.997092575

Topological polar surface area  1230.54

Aromaticity  0.129032258

Average rotatable bonds  3.516129032

Number of hydrogen bond donors  45

Number of hydrogen bond acceptors  43

X logP energy  -8.61476

Hydropathy 



Literature Information


Title  Enhanced Extracellular Production and Characterization of Sucrose Isomerase in Bacillus subtilis with Optimized Signal Peptides

DOI  10.3390/foods11162468,

Publication Year  2022

PubMed ID  36010467,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.