SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB06314

Signal sequence   MKKGIIRFLLVSFVLFFALSTGITGVQAAPA

Length  31

Organism  Bacillus subtilis

UniProt ID  NA,

Motif  1..31



Activity Information


Pathway  NA

Natural/Artificial  Artificial

Subcellular location  Extracellular environment

Secretion efficiency  NA

Type  NA



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB06314.cif

Structure  SPKB06314.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  3297.04844

Aliphatic index  129.0322581

Isoelectric point  11.82305527

Charge (pH=7)   2.99739092

Topological polar surface area  1165.48

Aromaticity  0.129032258

Average rotatable bonds  3.451612903

Number of hydrogen bond donors  41

Number of hydrogen bond acceptors  41

X logP energy  -6.03013

Hydropathy 



Literature Information


Title  Enhanced extracellular recombinant keratinase activity in Bacillus subtilis SCK6 through signal peptide optimization and site-directed mutagenesis

DOI  10.1039/c9ra07866e,

Publication Year  2019

PubMed ID  35529123,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.