SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB06313

Signal sequence   MVSIRRSFEAYVDDMNIITVLIPAEQKEIM

Length  30

Organism  Bacillus subtilis

UniProt ID  NA,

Motif  1..30



Activity Information


Pathway  NA

Natural/Artificial  Artificial

Subcellular location  Extracellular environment

Secretion efficiency  NA

Type  NA



Structure Information


PDB ID  2E8Y,

Predicted Structure ID  SPKB06313.cif

Structure  SPKB06313.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  3513.14294

Aliphatic index  113.6666667

Isoelectric point  4.264691876

Charge (pH=7)   -1.996949435

Topological polar surface area  1401.85

Aromaticity  0.066666667

Average rotatable bonds  3.966666667

Number of hydrogen bond donors  48

Number of hydrogen bond acceptors  48

X logP energy  -9.59596

Hydropathy 



Literature Information


Title  Enhanced extracellular recombinant keratinase activity in Bacillus subtilis SCK6 through signal peptide optimization and site-directed mutagenesis

DOI  10.1039/c9ra07866e,

Publication Year  2019

PubMed ID  35529123,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.