SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB06311

Signal sequence   MAKPLSKGGILVKKVLIAGAVGTAVLFGTLSSGIPGLPAADA

Length  42

Organism  Bacillus subtilis

UniProt ID  NA,

Motif  1..42



Activity Information


Pathway  Sec

Natural/Artificial  Artificial

Subcellular location  Extracellular environment

Secretion efficiency  NA

Type  Sec/SPI



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB06311.cif

Structure  SPKB06311.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  3990.84424

Aliphatic index  127.8571429

Isoelectric point  10.80270632

Charge (pH=7)   2.997250648

Topological polar surface area  1472.58

Aromaticity  0.023809524

Average rotatable bonds  3.095238095

Number of hydrogen bond donors  50

Number of hydrogen bond acceptors  54

X logP energy  -12.8833

Hydropathy 



Literature Information


Title  Enhanced extracellular recombinant keratinase activity in Bacillus subtilis SCK6 through signal peptide optimization and site-directed mutagenesis

DOI  10.1039/c9ra07866e,

Publication Year  2019

PubMed ID  35529123,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.