SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB06273

Signal sequence   MKKKKTWKRFLHFSSAALAAGLIFTSAAPAEA

Length  32

Organism  Bacillus subtilis

UniProt ID  P94522,

Motif  1..32



Activity Information


Pathway  Sec

Natural/Artificial  Artificial

Subcellular location  Extracellular environment

Secretion efficiency  NA

Type  Sec/SPI



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB06273.cif

Structure  SPKB06273.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  3479.14684

Aliphatic index  73.75

Isoelectric point  11.36666267

Charge (pH=7)   5.089190032

Topological polar surface area  1331.55

Aromaticity  0.125

Average rotatable bonds  3.59375

Number of hydrogen bond donors  48

Number of hydrogen bond acceptors  47

X logP energy  -10.40913

Hydropathy 



Literature Information


Title  Optimal secretion of thermostable Beta-glucosidase in Bacillus subtilis by signal peptide optimization

DOI  10.1016/j.pep.2021.105843,

Publication Year  2021

PubMed ID  33631310,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.